Experimental Study on Low-Detection Limit for Immunomagnetic Reduction Assays by Manipulating Reagent Entities

Author

Yang, S.Y. ; Yang, C.C. ; Horng, H.E. ; Shih, B.Y. ; Chieh, J.J. ; Hong, C.Y. ; Yang, H.C.

Author_Institution

MagQu Co., Ltd., Taipei, Taiwan

Volume

12

Issue

2

fYear

2013

fDate

Jun-13

Firstpage

65

Lastpage

68

Abstract

The low limit of detection (LLD) plays an important role in biomolecular assays, especially for early-stage assays. Biomolecular detections usually involve the use of two main elements: a reagent and an analyzer, which both greatly contribute to the LLD. In this work, the relationships among the LLD and reagent-related factors are investigated. The to-be-detected biomolecule is c-reactive protein (CRP) as an example. The assay method is immunomagnetic reduction (IMR). The components of reagent are Fe₃O₄ magnetic nanoparticles bio-functionalized with antibodies against CRP, dispersed in pH-7.4 phosphate buffered saline solution. Several key factors of the reagent, such as particle concentration, volume ratio of reagent to sample, and particle size, are manipulated to optimize the LLD of detecting CRP.

Keywords

biochemistry; biological techniques; iron compounds; molecular biophysics; nanomagnetics; nanoparticles; pH; proteins; CRP detection; Fe₃O₄; IMR assays; LLD; analyzer; antibodies; biomolecular assays; biomolecular detections; c-reactive protein; early-stage assays; experimental study; immunomagnetic reduction assays; low-detection limit; magnetic nanoparticles; pH-7.4; particle concentration; particle size; phosphate buffered saline solution; reagent components; reagent entity manipulation; reagent factors; reagent volume ratio; Immune system; Magnetic resonance; Magnetic separation; Magnetic susceptibility; Magnetometers; Nanoparticles; Saturation magnetization; Immunomagnetic reduction; magnetic nanoparticles; reagent; Antibodies; C-Reactive Protein; Immunoassay; Indicators and Reagents; Limit of Detection; Magnetic Phenomena; Magnetite Nanoparticles; Particle Size; Sodium Chloride;

fLanguage

English

Journal_Title

NanoBioscience, IEEE Transactions on

Publisher

ieee

ISSN

1536-1241

Type

jour

DOI

10.1109/TNB.2013.2240009

Filename

6451290