DocumentCode :
472518
Title :
Label-free electrical detection of antigens based on micro-channel gating
Author :
Javanmard, Mehdi ; Nemat-Gorgani, Mohsen ; Talasaz, AmirAli H. ; Pease, Fabian ; Ronaghi, Mostafa ; Davis, Ronald W.
Author_Institution :
Stanford Univ., Stanford
fYear :
2008
fDate :
12-14 Feb. 2008
Firstpage :
8
Lastpage :
12
Abstract :
Current microbiological techniques used for multi-analyte detection, necessary for medical diagnostics, involve expensive and time consuming methods based on sandwich immunoassays. Described below is the design, fabrication, and testing of a rapid and inexpensive sensor, involving the use of microelectrodes in a micro-channel, which can be used to detect target antigens electrically (label-free format) in real-time. As a proof of principle, we have successfully demonstrated real-time detection of target antigens by measuring instantaneous changes in ionic impedance. We have also demonstrated the selectivity of our sensors in responding to target antigens while remaining irresponsive to non-target antigens. Using this technique, an array of these sensors can be multiplexed onto a biochip and used to detect the various biomarkers present in a complex mixture.
Keywords :
biomedical electrodes; biosensors; chemical variables measurement; microelectrodes; microfluidics; microsensors; patient diagnosis; antigen detection; biochip; biomarker; early cancer detection; electrical impedance biosensor; ionic impedance; label-free antigen electrical detection; medical diagnostics; microbiological techniques; microchannel gating; microelectrodes; microfluidic biosensor; multianalyte detection; Biomarkers; Biosensors; Fabrication; Immune system; Impedance measurement; Medical diagnosis; Microelectrodes; Sensor arrays; Sensor phenomena and characterization; Testing; antigen detection; early cancer detection; electrical impedance biosensor; microfluidic biosensor;
fLanguage :
English
Publisher :
iet
Conference_Titel :
Sensors Applications Symposium, 2008. SAS 2008. IEEE
Conference_Location :
Atlanta, GA
Print_ISBN :
978-1-4244-1962-3
Electronic_ISBN :
978-1-4244-1963-0
Type :
conf
Filename :
4472933
Link To Document :
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