Real time simulation of heat detection in DNA Thermosequencing

Author

Esfandyarpour, Hesaam ; Zheng, Bo ; Pease, R. Fabian W ; Davis, Ronald W.

Author_Institution

Stanford Univ., Stanford

fYear

2008

fDate

12-14 Feb. 2008

Firstpage

89

Lastpage

94

Abstract

In this paper we study enzymatic and heat profile modeling for Thermosequencing [1-5], and discuss the real time 2D and 3D finite element simulation results for chemical concentrations involved in the detection system. The modeling simulates not only the heat released through the DNA polymerization reaction and its corresponding temperature change, but also the heat and sensing efficiencies of different geometries and platform structures. The thermodynamic effects of a DNA coated bead are investigated. Then, based on heat diffusion results, we recommend a modified gated structure for the microfluidic detection platform by using control valves and show how this new platform could dramatically improve the detection efficiency. Finally, we discuss potential advantages and disadvantages DNA Thermosequencing in real world applications.

Keywords

DNA; enzymes; finite element analysis; microfluidics; polymerisation; thermodynamics; DNA coated bead thermodynamic effect; DNA polymerization reaction; DNA thermosequencing; enzymatic modeling; finite element simulation; heat detection; heat diffusion; microfluidic detection platform; profile modeling; real time simulation; Chemicals; DNA; Finite element methods; Geometry; Microfluidics; Polymers; Real time systems; Solid modeling; Temperature sensors; Thermodynamics; DNA sequencing; Microfluidics; Thermal sensing; Thermosequencing; biosensor;

fLanguage

English

Publisher

iet

Conference_Titel

Sensors Applications Symposium, 2008. SAS 2008. IEEE

Conference_Location

Atlanta, GA

Print_ISBN

978-1-4244-1962-3

Electronic_ISBN

978-1-4244-1963-0

Type

conf

Filename

4472950