Title :
The Modelling and Control of Fractionation Processes for Enzyme and Protein Purification
Author :
Niktari, M. ; Richardson, P. ; Ravenhall, R. ; Flanagan, M.T. ; Molloy, J. ; Hoare, M.
Author_Institution :
SERC Centre for Biochemical Engineering, Department of Chemical and Biochemical Engineering, University College London, Torrington Place, London WC1E 7JE, U.K.
Abstract :
The use of an automated microcentrifuge and flow injection analyser is described for the measurement on-line of the solubility profiles and the resultant fractionation diagram for the purification by salting-out of enzymes from a microbial cell homogenate. The use of this system is demonstrated for the on-line control of the fractional precipitation of alcohol dehydrogenase from a clarified yeast homogenate using ammonium sulphate.
Keywords :
Biochemistry; Cells (biology); Control systems; Educational institutions; Fractionation; Particle measurements; Process control; Proteins; Purification; Solvents;
Conference_Titel :
American Control Conference, 1989
Conference_Location :
Pittsburgh, PA, USA
