Title :
Construction of Ribavirin Engineering Bacteria
Author :
Xing, Chen-Guang ; Chen, Ning ; Xie, Xi-Xian ; Zhao, Xi-Jing ; Xu, Qing-Yang
Author_Institution :
Coll. of Biotechnol., Tianjin Univ. of Sci. & Technol., Tianjin, China
Abstract :
In this study, the gene encoding purine nucleoside phosphorylase (PNPase) from Bacillus subtilis W168 was identified, cloned and expressed in Bacillus subtilis AG208. The gene encodes a polypeptide of 233 amino acids with a calculated molecular weight of 25,018 Da. The enzyme activity of the recombinant protein (AGPNP) was analyzed by temperature and pH perturbation difference spectra. Results showed that the optimum of temperature was 65degC and the optimum pH was 7.5 and the PNPase activity of the recombinant bacteria was increased by 193.9%.
Keywords :
biochemistry; biothermics; drugs; enzymes; genetics; microorganisms; molecular biophysics; proteins; Bacillus subtilis AG208; Bacillus subtilis W168; amino acids; bacteria; enzyme activity; gene encoding; pH effect; polypeptide; protein thermostability; purine nucleoside phosphorylase; recombinant protein; ribavirin; Biochemistry; Chemicals; Cloning; DNA; Electronic mail; Microorganisms; Pharmaceutical technology; Production; Proteins; Temperature; Bacillus subtilis; purine nucleoside phosphorylase; ribavirin;
Conference_Titel :
Environmental Science and Information Application Technology, 2009. ESIAT 2009. International Conference on
Conference_Location :
Wuhan
Print_ISBN :
978-0-7695-3682-8
DOI :
10.1109/ESIAT.2009.50
