Fiber-optic endomicroscopy for intrinsic two-photon fluorescence imaging of epithelial cells and tissues

Author

Wu, Yicong ; Xi, Jiefeng ; Chen, Yongping ; Cobb, Michael J. ; Li, Min-Jun ; Li, Xingde

Author_Institution

Dept. of Bioeng., Univ. of Washington, Seattle, WA, USA

fYear

2009

fDate

2-4 June 2009

Firstpage

1

Lastpage

2

Abstract

An endomicroscope with enhanced signal collection efficiency was developed using customized double-clad fiber and aspherical compound-lens. Ex vivo two-photon fluorescence imaging of epithelial tissues was demonstrated for the first time with an all-fiber-optic scanning endomicroscope.

Keywords

biological tissues; biomedical optical imaging; cellular biophysics; endoscopes; fluorescence spectroscopy; medical image processing; two-photon spectroscopy; all-fiber-optic scanning endomicroscopy; aspherical compound-lens; customized double-clad fiber; epithelial cells; intrinsic two-photon fluorescence imaging; signal collection efficiency; tissues; Biomedical optical imaging; Fiber nonlinear optics; Fluorescence; Lenses; Nonlinear optics; Optical beams; Optical imaging; Optical microscopy; Optical scattering; Optical sensors; (170.2150) Endoscopic imaging; (190.4180) Multiphoton processes;

fLanguage

English

Publisher

ieee

Conference_Titel

Lasers and Electro-Optics, 2009 and 2009 Conference on Quantum electronics and Laser Science Conference. CLEO/QELS 2009. Conference on

Conference_Location

Baltimore, MD

Print_ISBN

978-1-55752-869-8

Electronic_ISBN

978-1-55752-869-8

Type

conf

Filename

5224360