Three-dimensional super-resolution single-molecule fluorescence imaging using a double-helix point spread function

Author

Thompson, Michael A. ; Biteen, Julie S. ; Moerner, W.E. ; Pavani, Sri Rama Prasanna ; Piestun, Rafael

Author_Institution

Dept. of Chem., Stanford Univ., Stanford, CA, USA

fYear

2009

fDate

2-4 June 2009

Firstpage

1

Lastpage

2

Abstract

Fluorescence imaging with resolution ten times better than the diffraction limit in three dimensions over a depth of field of 2 mum is demonstrated with a widefield microscope that exhibits a double-helix point spread function.

Keywords

fluorescence; image resolution; light diffraction; optical images; optical microscopy; optical transfer function; 3D superresolution; depth 2 mum; diffraction limit; double-helix point spread function; fluorescence microscopy; optical transfer function; single-molecule fluorescence imaging; widefield microscopy; Biomedical optical imaging; Fluorescence; Image resolution; Lenses; Optical diffraction; Optical imaging; Optical microscopy; Signal resolution; Spatial resolution; Transfer functions; (100.6640) Superresolution; (110.4850) Optical transfer functions; (180.6900) Three-dimensional microscopy;

fLanguage

English

Publisher

ieee

Conference_Titel

Lasers and Electro-Optics, 2009 and 2009 Conference on Quantum electronics and Laser Science Conference. CLEO/QELS 2009. Conference on

Conference_Location

Baltimore, MD

Print_ISBN

978-1-55752-869-8

Electronic_ISBN

978-1-55752-869-8

Type

conf

Filename

5224379