Two-wavelength quantitative phase unwrapping of dynamic biological processes

Author

Rinehart, Matthew T. ; Shaked, Natan T. ; Wax, Adam

Author_Institution

Fitzpatrick Inst. for Photonics, Duke Univ., Durham, NC, USA

fYear

2009

fDate

2-4 June 2009

Firstpage

1

Lastpage

2

Abstract

A two-wavelength phase unwrapping technique is implemented on two different phase microscopy systems developed in our laboratory: asynchronous and dynamic phase microscopes. Both systems are based on digital holography interferometric setups and in both systems one acquires two phase shifted interferograms to eliminate common phase noise. However, in the first system the two phase-shifted interferograms are separated in time, whereas in the second system they are separated in space. Asynchronous digital holography uses two near- simultaneous phase-shifted interferograms to recover phase information.

Keywords

holographic interferometry; optical images; optical microscopy; phase shifting interferometry; asynchronous digital holography; asynchronous phase microscopic technique; dynamic phase microscopic technique; phase information recovery; phase shifted interferogram; quantitative phase imaging; two-wavelength quantitative phase unwrapping system; Apertures; Biological processes; Biomedical optical imaging; Holographic optical components; Holography; Lighting; Microscopy; Optical interferometry; Optical polarization; Phase shifting interferometry; (110.5086) Phase Unwrapping; (180.3170) Interference Microscopy;

fLanguage

English

Publisher

ieee

Conference_Titel

Lasers and Electro-Optics, 2009 and 2009 Conference on Quantum electronics and Laser Science Conference. CLEO/QELS 2009. Conference on

Conference_Location

Baltimore, MD

Print_ISBN

978-1-55752-869-8

Electronic_ISBN

978-1-55752-869-8

Type

conf

Filename

5225111