Title :
Real-time studies of muscle cell contractions with second harmonic generation microscopy
Author :
Prent, N. ; Cisek, R. ; Greenhalgh, C. ; Major, A. ; Stewart, B. ; Barzda, V.
Author_Institution :
Dept. of Phys., Univ. of Toronto, Mississauga, ON, Canada
Abstract :
Real-time second harmonic generation microscopy of live myocytes was achieved with a femtosecond diode-pumped Yb:KGd(WO4)2 laser. Negligible absorption allows for prolonged imaging during muscle contractions with no significant phototoxic effects or signal degradation.
Keywords :
bio-optics; cellular biophysics; high-speed optical techniques; laser applications in medicine; muscle; optical harmonic generation; semiconductor lasers; femtosecond diode pumped laser; muscle cell contractions; myocytes; phototoxic effects; second harmonic generation microscopy; signal degradation; Absorption; Biomedical optical imaging; Diodes; Frequency conversion; Muscles; Nonlinear optics; Optical harmonic generation; Optical imaging; Optical microscopy; Ultrafast optics; (140.3615) Lasers, Ytterbium; (170.3880) Medical and Biological Imaging;
Conference_Titel :
Lasers and Electro-Optics, 2009 and 2009 Conference on Quantum electronics and Laser Science Conference. CLEO/QELS 2009. Conference on
Conference_Location :
Baltimore, MD
Print_ISBN :
978-1-55752-869-8
Electronic_ISBN :
978-1-55752-869-8
