Fluorescence monitoring of microchip capillary electrophoresis separation with monolithically integrated optical waveguides

Author

Dongre, C. ; Dekker, R. ; Hoekstra, H.J.W.M. ; Martinez-Vazquez, R. ; Osellame, R. ; Ramponi, R. ; Cerullo, G. ; van Weeghel, R. ; Besselink, G. A J ; Van den Vlekkert, H.H. ; Pollnau, M.

Author_Institution

Integrated Opt. Microsyst. (IOMS), Univ. of Twente, Enschede, Netherlands

fYear

2009

fDate

2-4 June 2009

Firstpage

1

Lastpage

2

Abstract

Femtosecond-laser-written waveguides were integrated with fluidic microchannels in a commercial lab-on-a-chip. High-spatial-resolution monitoring of fluorescently labeled DNA molecules during capillary electrophoresis separation is demonstrated, as an intermediate step toward point-of-care diagnostic bioassays for DNA analysis.

Keywords

DNA; bioMEMS; biosensors; electrophoresis; fluorescence; high-speed optical techniques; integrated optics; lab-on-a-chip; laser materials processing; micro-optomechanical devices; microchannel flow; microsensors; molecular biophysics; optical sensors; optical waveguides; DNA analysis; biological sensor; femtosecond-laser-written waveguide; fluidic microchannel; fluorescence monitoring; fluorescently labeled DNA molecule; high-spatial-resolution monitoring; lab-on-a-chip; laser material processing; microchip capillary electrophoresis separation; monolithically integrated optical waveguide; optical sensor; point-of-care diagnostic bioassay; Biomedical optical imaging; DNA; Electrokinetics; Fluidic microsystems; Fluorescence; Integrated optics; Microfluidics; Monitoring; Optical waveguides; Ultrafast optics; (130.2755) Glass waveguides; (130.3120) Integrated optics devices; (140.3390) Laser materials processing; (260.2510) Fluorescence; (280.1415) Biological sensing and sensors; (280.4788) Optical sensing and sensors;

fLanguage

English

Publisher

ieee

Conference_Titel

Lasers and Electro-Optics, 2009 and 2009 Conference on Quantum electronics and Laser Science Conference. CLEO/QELS 2009. Conference on

Conference_Location

Baltimore, MD

Print_ISBN

978-1-55752-869-8

Electronic_ISBN

978-1-55752-869-8

Type

conf

Filename

5226155