Biosorption Characteristics of Pseudomonas Fluorescens PFC02 Biomass for Removal of Pb(II) Ions from Aqueous Solution

The Pb(II) biosorption characteristics of Pseudomonas fluorescens PFC02 biomass was examined as a function of initial pH, contact time and initial metal ion concentration. FTIR spectra showed that the principal functional sites taking part in the sorption process included carboxyl , hydroxyl groups and -CH3 stretching groups, the mechanism analysis showed that the chemical chelating was the main adsorption form, electrostatic attraction hydrogen bonding and van der Waals forced all function in the process of adsorption. The pseudo-second-order kinetic model was found to be well suited for the entire adsorption process of Pb(II) on PFC02. Adsorption equilibrium studies showed that Pb(II) adsorption data followed the Langmuir model, the maximum binding capacity of Pb(II) according to Langmuir isotherm were 80.00 mg/g at pH 7.0 , shaker speed 150 rpm, at 27 ¿C and for 60 min. The present study indicated that PFC02 biomass may be used as an inexpensive, effective and easily cultivable biosorbent for the removal of Pb(II) ions from environmental and industrial wastewater.