Discrimination of normal myometrial tissues, hysteromyoma and endometrial carcinoma with HATR-FTIR spectroscopy

Normal myometrial tissues, hysteromyoma and endometrial carcinoma were determined by horizontal attenuated total reflectance Fourier transform infrared spectroscopy (HATR-FTIR) directly and fast. The results showed that there are obvious and regularity differences HATR-FTIR spectra of them in spectral parameters such as frequency, intensity and shape of the bands. They indicated significant differences of content, structure and conformation of proteins, nucleic acids and lipids in normal myometrial tissues, hysteromyoma and endometrial carcinoma. Through the criticisms of goodness-of-fit tests of frequency of the bands in the second derivative FTIR, between each of the normal tissue samples and endometrial carcinoma, the probability is less than 0.01 and the result is significant. The present results suggested that HATR-FTIR could show the properties of normal myometrial tissues, hysteromyoma and endometrial carcinoma at the molecular level. It was able to supply rich and reliable information to investigation of benign tumor and cancer of uterine body, and will be used as a convenient and reliable diagnostic tool for some tumors.