A microfluidic system for semicontinuous bead-based biosensing platforms

Author

Kim, Hye Won ; Kim, Young Man ; Yoo, Sung Keun ; Yoon, Sang Youl ; Lee, Jong Hyun ; Yang, Sung

Author_Institution

Department of Mechatronics, 261 Cheomdan-gwagiro (Oryong-dong), Buk-gu, Gwangju, Korea 500-712

Volume

2009-Supplement

fYear

2009

fDate

2-6 Nov. 2009

Firstpage

1

Lastpage

6

Abstract

Conventional bead-based micro immunoassays do not guarantee high reliability and repeatability due to the trapping mechanisms of the systems which cause inconsistency in trapping of cytometric beads. This paper presents a new beadbased micro immunoassay system for semicontinuous assays with high reliability and accuracy in cytometric bead trapping by using the hole-patterned microstructures. Trapping test showed the assembly of single layer of bead array in ordered fashion. In addition that, the µ-immunoassay exhibits that the fluorescence intensity linearly increases in proportion to Biotin-4-Fluorescin (B4F) concentration and a 10 pg/mL appears to be the lowest detectable concentration for B4F after incubation time of 5 minutes.

Keywords

Biosensors; Microfluidics; Bead-based microimmunoassay; cytometric bead trapping; microfluidic device;

fLanguage

English

Publisher

ieee

Conference_Titel

Communications and Photonics Conference and Exhibition (ACP), 2009 Asia

Conference_Location

Shanghai, China

Print_ISBN

978-1-55752-877-3

Electronic_ISBN

978-1-55752-877-3

Type

conf

Filename

5405578