Title :
High throughput photoporation of mammalian cells using Microfluidic Cell Delivery
Author :
Marchington, Robert F. ; Arita, Yoshihiko ; Stevenson, David J. ; Gunn-Moore, Frank J. ; Dholakia, Kishan
Author_Institution :
SUPA, Univ. of St Andrews, St. Andrews, UK
Abstract :
Photoporation (optical injection) of mammalian cells using a tightly focused femtosecond laser beam is demonstrated within a microfluidic chip, providing delivery of cells to the beam and thus automating the system for high cell throughput.
Keywords :
high-speed optical techniques; laser beams; microfluidics; multiphoton processes; high throughput photoporation; mammalian cells; microfluidic cell delivery; microfluidic chip; multiphoton processes; optical injection; tightly focused femtosecond laser beam; ultrafast lasers; Biomedical optical imaging; Biomembranes; Cells (biology); Hydrodynamics; Laser beams; Microfluidics; Optical pulses; Optical pumping; Throughput; Ultrafast optics;
Conference_Titel :
Lasers and Electro-Optics (CLEO) and Quantum Electronics and Laser Science Conference (QELS), 2010 Conference on
Conference_Location :
San Jose, CA
Print_ISBN :
978-1-55752-890-2
Electronic_ISBN :
978-1-55752-890-2
