Title :
High-resolution fluorescence microscopy employing a cyclic sequential multiphoton excitation
Author :
Isobe, Keisuke ; Suda, Akira ; Hashimoto, Hiroshi ; Kannari, Fumihiko ; Kawano, Hiroyuki ; Mizuno, Hideaki ; Miyawaki, Atsushi ; Midorikawa, Katsumi
Author_Institution :
Laser Technol. Lab., RIKEN Adv. Sci. Inst., Wako, Japan
Abstract :
We demonstrate high-resolution fluorescence microscopy based on a cyclic sequential multiphoton process, which gives rise to fluorescence emission following a sequence of cyclic transitions between the bright and dark states of a fluorophore.
Keywords :
excited states; fluorescence; multiphoton processes; optical microscopy; cyclic sequential multiphoton excitation; fluorescence emission; fluorophore; high resolution fluorescence microscopy; Biological information theory; Biomedical optical imaging; Electron microscopy; Fluorescence; Optical diffraction; Optical microscopy; Optical pumping; Optical saturation; Spatial resolution; Stimulated emission;
Conference_Titel :
Lasers and Electro-Optics (CLEO) and Quantum Electronics and Laser Science Conference (QELS), 2010 Conference on
Conference_Location :
San Jose, CA
Print_ISBN :
978-1-55752-890-2
Electronic_ISBN :
978-1-55752-890-2
