DocumentCode
520151
Title
Double-helix microscopy for wide-field 3D single-molecule fluorescence imaging
Author
Sharma, Ginni ; Pavani, Rama Prasanna Prasanna ; Quirin, Sean ; Piestun, Rafael
Author_Institution
Dept. of Electr., Comput. & Energy Eng., Univ. of Colorado, Boulder, CO, USA
fYear
2010
fDate
16-21 May 2010
Firstpage
1
Lastpage
2
Abstract
We present methods to improve the localization accuracy in wide-field 3D single-molecule double-helix microscopy. We analyze the optical efficiency of the system, the fundamental limit for 3D localization, the estimation algorithms, and polarization sensitive detection.
Keywords
fluorescence; light polarisation; optical microscopy; 3D localization; double helix microscopy; estimation algorithms; localization accuracy; polarization sensitive detection; wide field 3D single molecule fluorescence imaging; DH-HEMTs; Data mining; Fluorescence; Focusing; Lenses; Microscopy; Optical imaging; Optical sensors; Polarization; Yield estimation;
fLanguage
English
Publisher
ieee
Conference_Titel
Lasers and Electro-Optics (CLEO) and Quantum Electronics and Laser Science Conference (QELS), 2010 Conference on
Conference_Location
San Jose, CA
Print_ISBN
978-1-55752-890-2
Electronic_ISBN
978-1-55752-890-2
Type
conf
Filename
5499714
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