Effect of electric field on activity index of Horseradish peroxidase

Author

Yang, Zhihuai ; Wang, Miao ; Xu, Qiang

Author_Institution

Dept. of Phys., Baoji Univ. of Arts & Sci., Baoji, China

Volume

3

fYear

2010

fDate

16-18 Oct. 2010

Firstpage

1303

Lastpage

1306

Abstract

The effect of electric field on the activity index of Horseradish peroxidase (HRP) was investigated by a combination of Ultraviolet-visible, FT-infrared and Circular Dichroism spectroscopic techniques. The result showed that in comparison with the control, electric field could increase the activity index of HRP; Based on these changes, it can be concluded that the electric field could affect charge distribution of group of the amides in the polypeyide chains of HRP and thus cause the change in the secondary structure of HRP. It would change in the structure of the porphyrin group in the heme group of HRP and lead the change in the activity index of HRP. The result of this study has important meaning to explain the biological effect of electric treatment seeds.

Keywords

biological effects of fields; biomedical measurement; biomolecular effects of radiation; circular dichroism; electric field effects; enzymes; infrared spectra; molecular configurations; ultraviolet spectra; visible spectra; FT-infrared spectra; Horseradish peroxidase; activity index; amide group; charge distribution; circular dichroism; electric field effect; electric treatment seeds; heme group; polypeyide chains; porphyrin group; secondary structure; ultraviolet-visible spectra; Absorption; Electric fields; Indexes; Proteins; Spectroscopy; Activity index; Electric field; Horseradish peroxidase; Secondary structure;

fLanguage

English

Publisher

ieee

Conference_Titel

Biomedical Engineering and Informatics (BMEI), 2010 3rd International Conference on

Conference_Location

Yantai

Print_ISBN

978-1-4244-6495-1

Type

conf

DOI

10.1109/BMEI.2010.5639250

Filename

5639250