Temperature dependence of ionic current in solid-state nanopore

As the new generation DNA sequencing technology, nanopore sequencing has demonstrated several advantages of low-cost and high-throughout, contrasting to present commercial sequencing methods. The temperature is an influential factor in the nanopore sequencing system. This paper presents the temperature control system based on the pulse-width modulation (PWM) technique. And the accuracy of the temperature control system can reach 0.3°C. By controlling the electrolyte temperature, KCl concentration on the nanopore, we obtain that the ionic current is positively associated with them.