Biotransformation of digoxigenin by Arthrobacter simplex TCCC 11037

Author

Liu, Zengbing ; Sun, Hua ; Zheng, Yu ; He, Chaojun ; Fu, Yuwan ; Wang, Min

Author_Institution

Key Lab. of Ind. Microbiol., Minist. of Educ., Tianjin, China

Volume

5

fYear

2010

fDate

16-18 Oct. 2010

Firstpage

1997

Lastpage

2000

Abstract

Biotransformation of natural products has great potential for production of new drugs or Lead Compounds. In this paper biotransformation of digoxigenin (1) by Arthrobacter simplex TCCC 11037 was investigated. The biotransformation reaction was carried out in a 7-day fermentation process. Three products purified from broth culture supernatants by silica gel column chromatography. And they were identified as digoxigenone (2), 16(17)-ene-digoxigenone (3), and 1(2),16(17)-dien- digoxigenone (4) by FT-ICR-MS and NMR. This is the first report of the production of compound 2 and compound 3 by a biotransformation reaction.

Keywords

Fourier transform spectra; biochemistry; biotechnology; chemical reactions; chromatography; drugs; fermentation; mass spectra; microorganisms; molecular biophysics; nuclear magnetic resonance; organic compounds; 1(2),16(17)-dien- digoxigenone; 16(17)-ene-digoxigenone; Arthrobacter simplex TCCC 11037; FT-ICR-MS; NMR; biotransformation reaction; culture supernatants; digoxigenin biotransformation; fermentation process; silica gel column chromatography; time 7 day; Carbon; Chemicals; Drugs; Production; Purification; Silicon compounds; Arthrobacter simplex; biotransformation; digoxigenin; digoxin;

fLanguage

English

Publisher

ieee

Conference_Titel

Biomedical Engineering and Informatics (BMEI), 2010 3rd International Conference on

Conference_Location

Yantai

Print_ISBN

978-1-4244-6495-1

Type

conf

DOI

10.1109/BMEI.2010.5639441

Filename

5639441