The study on anti-tumor activity of Xinghuayu injection

Purpose: to study the anti-tumor effect and corresponding mechanism of Xinghuayu injection. Methods: (1) anti-tumor effect: Kunming mice were inoculated with hepatoma H₂₂ cells, and then were divided into model group, three dose Xinghuayu injection group and positive group, drug administrate red intraperitoneally once a day. After 7 days, the mice were killed to strip out the tumor blocks to weight. (2) synergistic action: take tumor-inoculated mice and divide them into Xinghuayu injection group, cyclophosphamide group and Xinghuayu combined cyclophosphamide group. All administration and other process was the same as that in (1). (3) measurement of serum IL-2 and TNF-α: take tumor-inoculated mice and divide them into model group, three dose Xinghuayu group and cyclophosphamide group. Administration to the above groups was the same as in (1). Take blood and detect the concentration of serum IL-2 and TNF-α. Results: (1) the tumor block weight of every Xinghuayu injection group is lower than model group, P <;; 0.01. (2) the tumor block weight of drug combination group is significantly lower than that of either single drug group, and the tumor inhibition ratio is significantly larger than either single drug group, P<;;0.05. (3) both of the serum IL-2 and TNF-α concentrations in middle and high dose Xinghuayu group are significantly larger than that of model group, P<;;0.01. Conclusion: Xinghuayu injection can inhibit tumor cell growth in tumor-bearing mice, and can enhance the anti-tumor effect of cyclophosphamide; Xinghuayu can increase the serum IL-2 and TNF-α concentration in tumor-bearing mice, suggesting that Xinghuayu´s anti-tumor effect is related with enhanced immune function.