Title :
Preparation of low-molecular peptides by enzyme autolysis of paphia undulate
Author :
Chen, Xin ; Chen, Xiaogang ; Zhou, Debo ; Sun, Huili ; Chen, Hua
Author_Institution :
Dept. of Chem. & Chem. Eng., Foshan Univ., Foshan, China
Abstract :
Paphia undulate was used to prepare low-molecular peptides by enzyme autolysis. The optimum condition for autolysis was studied to reach a maximum yield of peptides. Results showed that the yield of low-molecular peptides was 4.33% and 78.57% of the total protein can be recovered under the optimized condition: pH 6.5, temperature 45 Degrees Celsius, reaction time 4h, ratio of starting material and water 1:3 and the enzyme dosage of 1374.0 U/g.
Keywords :
biochemistry; enzymes; macromolecules; pH; enzyme autolysis; enzyme dosage; low-molecular peptides; pH 6.5; paphia undulate; starting material; temperature 45 C; total protein; Amino acids; Peptides; Proteins; Substrates; Temperature; Paphia undulat; enzyme autolysis; low-molecular peptides; optimum condition; orthogonal experiment; preparation;
Conference_Titel :
Biomedical Engineering and Informatics (BMEI), 2010 3rd International Conference on
Conference_Location :
Yantai
Print_ISBN :
978-1-4244-6495-1
DOI :
10.1109/BMEI.2010.5640021
