In vitro anti-hepatitis B virus activity of konjac oligo-glucomannan sulfate

Author

Huang, Hao ; Yang, Zhonghua ; Wang, Guanghui ; Chen, Jun

Author_Institution

Biol. Eng. Inst., Wu Han Univ. of Sci. & Technol., Wuhan, China

Volume

4

fYear

2010

fDate

16-18 Oct. 2010

Firstpage

1690

Lastpage

1694

Abstract

Glucomannan extracted from konjac was sulfated (konjac oligo-glucomannan sulfate, OGMS) to show selective anti-hepatitis B virus activity in vitro using HepG₂-2.2.15 cell line as the host cell of Hepatitis B virus. OGMS straight inhibited the secretion of HBsAg, HBeAg and HBV-DNA Replication in HepG₂-2.2.15 cell by ELISA and FQ-PCR. The studies indicated that OGMS mainly acted as an HBsAg inhibitor by and its IC₅₀ was 0.2675 mg/mL by Reed-Muench method; HBV-DNA Replication was inhibited as well. All these indicated that OGMS had evident in vitro anti-hepatitis B virus activity.

Keywords

biochemistry; cellular biophysics; microorganisms; molecular biophysics; patient treatment; pharmaceuticals; ELISA; FQ-PCR; HBV-DNA replication; HBeAg; HBsAg inhibitor; HepG₂-2.2.15 cell line; Konjac oligo-glucomannan sulfate; OGMS; Reed-Muench method; host cell; in vitro anti-hepatitis B virus activity; DNA; Drugs; Extracellular; In vitro; Plastics; Real time systems; Three dimensional displays; Hepatitis B virus; In vitro; Konjac; Polysaccharide sulfate;

fLanguage

English

Publisher

ieee

Conference_Titel

Biomedical Engineering and Informatics (BMEI), 2010 3rd International Conference on

Conference_Location

Yantai

Print_ISBN

978-1-4244-6495-1

Type

conf

DOI

10.1109/BMEI.2010.5640540

Filename

5640540