Title :
In vitro anti-hepatitis B virus activity of konjac oligo-glucomannan sulfate
Author :
Huang, Hao ; Yang, Zhonghua ; Wang, Guanghui ; Chen, Jun
Author_Institution :
Biol. Eng. Inst., Wu Han Univ. of Sci. & Technol., Wuhan, China
Abstract :
Glucomannan extracted from konjac was sulfated (konjac oligo-glucomannan sulfate, OGMS) to show selective anti-hepatitis B virus activity in vitro using HepG2-2.2.15 cell line as the host cell of Hepatitis B virus. OGMS straight inhibited the secretion of HBsAg, HBeAg and HBV-DNA Replication in HepG2-2.2.15 cell by ELISA and FQ-PCR. The studies indicated that OGMS mainly acted as an HBsAg inhibitor by and its IC50 was 0.2675 mg/mL by Reed-Muench method; HBV-DNA Replication was inhibited as well. All these indicated that OGMS had evident in vitro anti-hepatitis B virus activity.
Keywords :
biochemistry; cellular biophysics; microorganisms; molecular biophysics; patient treatment; pharmaceuticals; ELISA; FQ-PCR; HBV-DNA replication; HBeAg; HBsAg inhibitor; HepG2-2.2.15 cell line; Konjac oligo-glucomannan sulfate; OGMS; Reed-Muench method; host cell; in vitro anti-hepatitis B virus activity; DNA; Drugs; Extracellular; In vitro; Plastics; Real time systems; Three dimensional displays; Hepatitis B virus; In vitro; Konjac; Polysaccharide sulfate;
Conference_Titel :
Biomedical Engineering and Informatics (BMEI), 2010 3rd International Conference on
Conference_Location :
Yantai
Print_ISBN :
978-1-4244-6495-1
DOI :
10.1109/BMEI.2010.5640540
