DocumentCode
544174
Title
Comparison of various activation methods of Sepharose CL-4B to optimize the stability of immunoadsorbents
Author
Ubrich, Nathalie ; Regnault, Véronique ; De Seyssel, Marc ; Rivat, Claude ; Hubert, Patrick ; Dellacherie, Edith
Author_Institution
INSERM, Vandoeuvre-Les-Nancy, France
Volume
1
fYear
1992
fDate
Oct. 29 1992-Nov. 1 1992
Firstpage
12
Lastpage
13
Abstract
During the use of chromatographic supports for the production of pure proteins or the cleansing of biological fluids by affinity chromatography, a leakage of ligands coupled to the matrix is generally observed. To study and minimize this one, a comparison of immunoadsorbents prepared with Sepharose CL-4B activated by various chemical reagents, and coupled to goat anti apoB polyclonal antibodies was performed.
Keywords
adsorption; biochemistry; chromatography; molecular biophysics; proteins; Sepharose CL-4B; activation method; affinity chromatography; biological fluids; chemical reagents; cleansing; goat antiapoB polyclonal antibodies; immunoadsorbents; proteins; Ethanol; Immune system; Neodymium; Voltage control;
fLanguage
English
Publisher
ieee
Conference_Titel
Engineering in Medicine and Biology Society, 1992 14th Annual International Conference of the IEEE
Conference_Location
Paris
Print_ISBN
0-7803-0785-2
Electronic_ISBN
0-7803-0816-6
Type
conf
DOI
10.1109/IEMBS.1992.5760833
Filename
5760833
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