Homogeneous fluorescence polarization immunoassay for eicosanoids

Author

Matt, M. ; Nicolas, A. ; Donner, M. ; Stoltz, J.F.

Author_Institution

Fac. des Sci. Pharm. et Biol., Lab. de Chim. Anal., Univ. de Nancy, Nancy, France

Volume

1

fYear

1992

fDate

Oct. 29 1992-Nov. 1 1992

Firstpage

93

Lastpage

94

Abstract

Homogeneous fluorescence polarization immunoassay (FPIA) was applied to the development of an immunoassay of prostaglandin E₁ (PGE₁) selected as a model of eicosanoids. PGE₁ was derivatized with fluoresceinthiocarbamyl ethylenediamine and purified by thin layer chromatography (TLC). The purity was checked by high performance liquid chromatography (HPLC). A radioimmunoassay was realized to ensure of the immunocompetition of the tracer. After the determination of the concentration of antiserum by fluorescence polarization, the feasability of the immunoassay was shown.

Keywords

bio-optics; chromatography; fluorescence; molecular biophysics; FPIA; antiserum; eicosanoids; fluoresceinthiocarbamyl ethylenediamine; high performance liquid chromatography; homogeneous fluorescence polarization immunoassay; immunocompetition; prostaglandin E₁; purity; radioimmunoassay; thin layer chromatography; Anisotropic magnetoresistance; Electronics packaging; Fluorescence;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology Society, 1992 14th Annual International Conference of the IEEE

Conference_Location

Paris

Print_ISBN

0-7803-0785-2

Electronic_ISBN

0-7803-0816-6

Type

conf

DOI

10.1109/IEMBS.1992.5760872

Filename

5760872