Quantitation of thrombogenicity of hemodialyzer with Indium-111 labeled platelets

Comparative platelet thrombogenicity was quantified with In-111 labeled platelets. The platelets collected from blood of Beagle dogs, Yorkshire pigs and human volunteer were labeled with detergent-free In-111 tropolon and sham-hemodialysis (SHD) was performed with hollow-fiber dialyzer (HD) in a flow-loop at 37°C, with flow-rates of 7-, 150- and 270 ml/min.; after SHD, HD-radioactivity was measured with an ionization chamber, gamma counter and imaged with a gamma camera. The mean values of HD-adherent platelet radioactivity were calculated. The canine platelets are more thrombogenic than porcine and human platelets. Thrombogenicity of porcine platelets is close to that of human platelets. The quantitation of comparative thrombogenicity with In-111 platelets may provide an estimation of prosthesis-induced thrombogenicity of human platelets from porcine platelets.