Title :
Detection of pathogens using bio-activated nickel hexacyanoferrate film coated interdigitated microelectrodes
Author :
Pires, N.M.M. ; Tao Dong ; Zhaochu Yang ; Karlsen, Frank
Author_Institution :
Dept. of Micro & Nano Syst. Technol., Vestfold Univ. Coll., Tonsberg, Norway
Abstract :
In the present work, the microfabricated impedance sensing array (MISA) unit, based on interdigitated microelectrodes (IDMEs) coated with nickel hexacyanoferrate (NiHCF) film, is presented as an alternative solution to detect pathogens. The NiHCF film, which provide a [Fe(CN)6]3-/4- probe-free redox reaction, was functionalized with hexahistine tags (6×His) antibodies with which they have the high-affinity. Variation in the interfacial impedance caused by bio-activated beads was measured to detect targeted Infectious Salmon Anaemia Virus (ISAV) antigen. A detection limit of 10pg/ml was demonstrated, which indicates that the MISA unit is promising to detect pathogens at early-stage of the infection.
Keywords :
food safety; microelectrodes; microorganisms; nickel; oxidation; probes; reduction (chemical); Ni; NiHCF; bioactivated nickel hexacyanoferrate film; infectious salmon anaemia virus antigen; interdigitated microelectrodes; microfabricated impedance sensing array; nickel hexacyanoferrate; pathogens detection; probe-free redox reaction; Electrodes; Films; Immune system; Impedance; Pathogens; Surface impedance; Surface treatment; bio-activated microbeads; interdigitated microelectrodes; interfacial impedance sensor; nickel hexacyanoferrate film; pathogen detection;
Conference_Titel :
Electronic and Mechanical Engineering and Information Technology (EMEIT), 2011 International Conference on
Conference_Location :
Harbin, Heilongjiang, China
Print_ISBN :
978-1-61284-087-1
DOI :
10.1109/EMEIT.2011.6023694
