H-MR spectroscopy quantification using LCModel with the simulated basis-set created by software Vespa

Magnetic Resonance Spectroscopy (MRS) is a non-invasive technique to measure in vivo metabolite concentration. However, an accurate and reliable quantification result is extremely essential. A commonly used commercial software for proton spectra processing is LCModel, which analyzes an observed in vivo spectrum from brain tissue as a Linear Combination of in vitro Model solutions spectra. The default model spectrum provided by LCModel is acquired from in vitro measurements of metabolic solutions using the same pulse sequence and scanning parameters as the in vivo measurement. In this study, we provide a new method to create a basis-set by Vespa-0.2.0 software simulation and compare the performance of default basis-set with the performance of a simulated one. Using Vespa simulation as the basis-set will provide adequate results as well. In vitro model solution collocation, which requires great care and expertise, will be avoided. A shortcut of basis-set production brings great convenience for various kinds of research.