Title :
Development and primary application of the SYBR Green I RT-qPCR assay for the detection of the transmissible gastroenteritis virus (TGEV) S gene
Author :
Gu, Jiangping ; Yue, Xiuwei ; Yuan, Congli ; Cui, Li ; Hua, Xiuguo ; Yang, Zhibiao ; Li, Caiying ; Xing, Rui
Author_Institution :
Shanghai Key Lab. of Veterinary Biotechnol., Shanghai Jiaotong Univ., Shanghai, China
Abstract :
This study established a SYBR Green I real-time quantitative PCR detection assay (RT-qPCR) for the detection of the porcine transmissible gastroenteritis virus (TGEV) using specific primers designed to amplify the highly conserved porcine TGEV S gene sequence. The threshold cycle (Ct) and the log plasmid copy numbers had a good linear relationship with an efficiency of 1.05 (R2 = 0.999). The advantages of utilizing this approach for the rapid detection of TGEV include excellent sensitivity, reproducibility, and low cost. Ninety-six porcine fecal samples were tested in this study, and 7 samples more than PCR assay were detected by this assay.
Keywords :
biomedical engineering; genomics; microorganisms; SYBR Green I RT-qPCR assay; TGEV S gene detection; log plasmid copy numbers; realtime quantitative PCR detection assay; threshold cycle; transmissible gastroenteritis virus detection; Diseases; Laboratories; Probes; Proteins; Real time systems; Sensitivity; Strain; RT-qPCR; S gene; SYBR Green I; TGEV;
Conference_Titel :
Biomedical Engineering and Informatics (BMEI), 2011 4th International Conference on
Conference_Location :
Shanghai
Print_ISBN :
978-1-4244-9351-7
DOI :
10.1109/BMEI.2011.6098527
