Improved detection limits of bacterial endotoxins using new type of planar interdigital sensors

Author

Syaifudin, A R Mohd ; Zia, Asif I. ; Mukhopadhyay, S.C. ; Yu, P.L. ; Gooneratne, Chinthaka P. ; Kosel, Jurgen

Author_Institution

Sch. of Eng. & Adv. Technol., Massey Univ., Palmerston North, New Zealand

fYear

2012

fDate

28-31 Oct. 2012

Firstpage

1

Lastpage

4

Abstract

New types of planar interdigital sensors were fabricated by photolithography and etching techniques on a Silicon/Silicon Dioxide (Si/SiO₂) wafer (single side polished). The sensors were then coated with APTES (3-aminopropyltrietoxysilane) a cross linker used to bind Polymyxin B (PmB) molecules on electrodes surface. PmB is an antimicrobial peptide produced by the Gram-positive bacterium-Bacillus which has specific binding properties to Lipopolysaccharide (LPS). This paper will discuss the fabrication process, coating and immobilization procedures and analysis of sensors´ performance based on Impedance Spectroscopy method. The sensor sensitivity was compared to standard ToxinSensor Chromogenic LAL Endotoxin Assay Kit for verification.

Keywords

biological techniques; cellular biophysics; chemical sensors; coating techniques; electrodes; etching; interdigital transducers; microorganisms; molecular biophysics; organic compounds; photolithography; 3-aminopropyltrietoxysilane; APTES; Gram-positive bacterium-Bacillus; LPS; PmB molecules; Si-SiO₂; antimicrobial peptide; bacterial endotoxin detection limit; bind polymyxin B; coating process; electrodes surface; etching technique; fabrication process; immobilization procedure; impedance spectroscopy method; lipopolysaccharide; photolithography; planar interdigital sensor; Electrodes; Gold; Impedance; Resists; Sensitivity; Sensors; Silicon;

fLanguage

English

Publisher

ieee

Conference_Titel

Sensors, 2012 IEEE

Conference_Location

Taipei

ISSN

1930-0395

Print_ISBN

978-1-4577-1766-6

Electronic_ISBN

1930-0395

Type

conf

DOI

10.1109/ICSENS.2012.6411193

Filename

6411193