Miniaturized label-free impedimetric immunosensor for analysis of aflatoxin B1 in peanut

Author

Bacher, G. ; Kanungo, L. ; Bhand, S.

Author_Institution

Dept. of EEE & I, BITS, Pilani, India

fYear

2012

fDate

18-21 Dec. 2012

Firstpage

29

Lastpage

35

Abstract

A highly sensitive and selective label-free impedimetric immunosensor based on silver (Ag) wire electrode for the detection aflatoxin B1 (AFB1) in peanut is presented. The sensor was constructed by functionalizing Ag wire coupled with polyclonal antibodies of AFB1 through self assembled monolayers. The antigen-antibody interaction was analyzed by measuring impedance in the frequency range (1-100 KHz) at 5mV applied ac potential in a 384 micro well plate with assay volume 105μL. A working range (0.01-100pg/mL) for AFB1 was obtained with 20min analysis time with limit of detection 0.01pg/mL. The obtained SD and R² values were 0.16 and 0.95 respectively.

Keywords

biosensors; electric impedance measurement; electrochemical electrodes; electrochemical sensors; molecular biophysics; monolayers; proteins; self-assembly; AFB1; Aflatoxin B1 detection; antigen-antibody interaction; frequency 1 kHz to 100 kHz; impedance measurement; miniaturized label free impedimetric immunosensor; peanut; polyclonal antibody; self assembled monolayer; time 20 min; voltage 5 mV; wire electrode; Electric potential; Electrodes; Equivalent circuits; Frequency measurement; Immune system; Impedance; Wires; AFB1; electrochemical impedance spectroscopy; immunosensor; label free; peanut;

fLanguage

English

Publisher

ieee

Conference_Titel

Sensing Technology (ICST), 2012 Sixth International Conference on

Conference_Location

Kolkata

ISSN

2156-8065

Print_ISBN

978-1-4673-2246-1

Type

conf

DOI

10.1109/ICSensT.2012.6461690

Filename

6461690