Title :
Lifetime map reconstruction in frequency-domain fluorescence lifetime imaging microscopy
Author :
Roudot, Philippe ; Kervrann, Charles ; Waharte, Francois ; Boulanger, Jerome
Author_Institution :
Inria Centre de Rennes - Bretagne Atlantique, Rennes, France
fDate :
Sept. 30 2012-Oct. 3 2012
Abstract :
We propose a robust statistical framework for reconstructing lifetime map corrupted by vesicle motion in frequency domain FLIM imaging. Instrumental noise is taken into account to improve lifetime estimation. Robust M-estimators and ML-estimators allow to jointly estimate motion and lifetime. Performances are demonstrated on simulated and real samples.
Keywords :
biomedical optical imaging; fluorescence; frequency-domain analysis; image reconstruction; medical image processing; Instrumental noise; ML-estimators; frequency domain FLIM imaging; frequency-domain fluorescence lifetime imaging microscopy; lifetime estimation; lifetime map reconstruction; robust M-estimators; robust statistical framework; vesicle motion; Charge coupled devices; Estimation; Microscopy; Noise; Photonics; Robustness; FLIM; M-estimation; fluorescence; frequency-domain; spot tracking; template matching;
Conference_Titel :
Image Processing (ICIP), 2012 19th IEEE International Conference on
Conference_Location :
Orlando, FL
Print_ISBN :
978-1-4673-2534-9
Electronic_ISBN :
1522-4880
DOI :
10.1109/ICIP.2012.6467415
