Title :
Autonomous operating process for zebrafish embryo injection
Author :
Wang Yiliao ; Sun Mingzhu ; Zhao Xin ; Zhao Baoquan
Author_Institution :
Inst. of Robot. & Autom. Inf. Syst., Nankai Univ., Tianjin, China
fDate :
Aug. 29 2012-Sept. 1 2012
Abstract :
Embryo injection is a significant technique in molecule biology and drug discovery for zebrafish, one of the most popular model organisms. Traditional zebrafish embyo injection is manually conducted under microscope, requiring challenging coordination of hand, eye and foot and their respective precise control as well as ceaseless labour. An autonomous operating process for zebrafish embryo injection is proposed in this paper. Based on a micromanipulation robot system, the operating process integrates computer vision and micromanipulation robot control to realize batch embryo injection automatically. Successful rate of 93% and survival ratio of 89.5% at operating speed of 6-7 embryos are obtained, verifying the feasibility and effectiveness of the process. Finally, the process is applied to investigate the toxicity of a dye and receives success.
Keywords :
cellular biophysics; drugs; dyes; eye; medical robotics; micromanipulators; robot vision; toxicology; zoology; autonomous operating process; computer vision; drug discovery; dye; eye; foot; hand; micromanipulation robot system; molecule biology; toxicity; zebrafish embryo injection; Embryo; Fluids; Microinjection; Microscopy; Robot kinematics; Autonomous Operating Process; Visual feedback; Zebrafish Embryo Injection;
Conference_Titel :
Manipulation, Manufacturing and Measurement on the Nanoscale (3M-NANO), 2012 International Conference on
Conference_Location :
Shaanxi
Print_ISBN :
978-1-4673-4588-0
Electronic_ISBN :
978-1-4673-4589-7
DOI :
10.1109/3M-NANO.2012.6472927
