Detection of base pairs in single-nucleotide polymorphisms (SNP) using a nanostructured biosensor

In this study, we report the effective detection of the mutation occurrence of allergy gene using a sensitive nanostructured biosensor based on an gold nanoparticles (GNPs) uniformly deposited sensing electrode. The MD2 expression related genome of 70 mer gene fragment was used as the probe for the detection of the gene mutation. Total number of 17 combined target samples, including 6 gene mutation samples and 11 normal samples, were used for the discrimination experiments. Experimental results illustrated that the nanostructured biosensor could clearly discriminate the mutations and non-mutations of the allergy genes of MD2 using the target samples prepared by a PCR process of 10 cycles.