Simulation of backward facing step flow at the outlet of micro-channel and optimal design of amperometric detection chip

In this paper, we discussed the vortex which cause by the step structure at the outlet of microchannel. When the the thickness of boundary layer and the height of the channel are fixed value, the vortex area length was decided by Reynolds number. COMSOL Multiphysics simulations based on the turbulence model were performed to provide a better understanding of the Reynolds number and the length of vortex area in the compound structure microfluidic amperometric detection chip. According to the simulation results, we discussed the value for the height of the microchannel and the thickness of auxiliary bonding layer, we determined the manufacture parameters and assembly process of the microfluidic chip. Furthermore through the simulation results discussion of the microfluidic properties, we described how to set experiment conditions for amperometric detection.