A capillary-based microfluidic device incorporating optical fibers for flow induced dispersion analysis

Author

Guisheng Zhuang ; Poulsen, Nicklas N. ; Petersen, Nickolaj J. ; Ostergaard, Jacob ; Jensen, H.

Author_Institution

Dept. of Pharmacy, Univ. of Copenhagen, Copenhagen, Denmark

fYear

2013

fDate

7-10 April 2013

Firstpage

1054

Lastpage

1057

Abstract

In this paper, we describe a capillary-based microfluidic device utilizing flow induced dispersion analysis (FIDA) for quantitative characterization of biomarkers. The microfluidic device is fabricated by micromilling technology and has incorporated buried optical fibers for light detection. The angle and distance between the fiber guiding the excitation light source and the fiber collecting fluorescent emission light were optimized to enhance signal-to-noise ratio (SNR) and limit of detection (LOD). The prototype achieves a LOD of 50 nM for the fluorescein indicator by using a low-cost Miniature Fiber Optic Spectrometer. The FIDA-based procedure employing fluorescein as the indicator and human serum albumin (HSA) as the analyte is carried out in the microfluidic device.

Keywords

capillarity; fibre optic sensors; fluorescence; micro-optomechanical devices; microfabrication; microfluidics; microsensors; milling; optical fibre dispersion; optical fibre fabrication; proteins; prototypes; spectrometers; FIDA; HSA analyte; LOD; SNR; biomarkers; buried optical fibers; capillary-based microfluidic device; detection limit; excitation light source; fiber guiding; flow induced dispersion analysis; fluorescein indicator; fluorescent emission light; human serum albumin; light detection; low-cost miniature fiber optic spectrometer; micromilling technology; optimization; prototype; signal-to-noise ratio; Computer vision; Decision support systems; Image motion analysis; Microfluidics; Optical fiber dispersion; Optical fibers; flow induced dispersion analysis; microfluidics; optical fibers;

fLanguage

English

Publisher

ieee

Conference_Titel

Nano/Micro Engineered and Molecular Systems (NEMS), 2013 8th IEEE International Conference on

Conference_Location

Suzhou

Electronic_ISBN

978-1-4673-6351-8

Type

conf

DOI

10.1109/NEMS.2013.6559903

Filename

6559903