Title :
A capillary-based microfluidic device incorporating optical fibers for flow induced dispersion analysis
Author :
Guisheng Zhuang ; Poulsen, Nicklas N. ; Petersen, Nickolaj J. ; Ostergaard, Jacob ; Jensen, H.
Author_Institution :
Dept. of Pharmacy, Univ. of Copenhagen, Copenhagen, Denmark
Abstract :
In this paper, we describe a capillary-based microfluidic device utilizing flow induced dispersion analysis (FIDA) for quantitative characterization of biomarkers. The microfluidic device is fabricated by micromilling technology and has incorporated buried optical fibers for light detection. The angle and distance between the fiber guiding the excitation light source and the fiber collecting fluorescent emission light were optimized to enhance signal-to-noise ratio (SNR) and limit of detection (LOD). The prototype achieves a LOD of 50 nM for the fluorescein indicator by using a low-cost Miniature Fiber Optic Spectrometer. The FIDA-based procedure employing fluorescein as the indicator and human serum albumin (HSA) as the analyte is carried out in the microfluidic device.
Keywords :
capillarity; fibre optic sensors; fluorescence; micro-optomechanical devices; microfabrication; microfluidics; microsensors; milling; optical fibre dispersion; optical fibre fabrication; proteins; prototypes; spectrometers; FIDA; HSA analyte; LOD; SNR; biomarkers; buried optical fibers; capillary-based microfluidic device; detection limit; excitation light source; fiber guiding; flow induced dispersion analysis; fluorescein indicator; fluorescent emission light; human serum albumin; light detection; low-cost miniature fiber optic spectrometer; micromilling technology; optimization; prototype; signal-to-noise ratio; Computer vision; Decision support systems; Image motion analysis; Microfluidics; Optical fiber dispersion; Optical fibers; flow induced dispersion analysis; microfluidics; optical fibers;
Conference_Titel :
Nano/Micro Engineered and Molecular Systems (NEMS), 2013 8th IEEE International Conference on
Conference_Location :
Suzhou
Electronic_ISBN :
978-1-4673-6351-8
DOI :
10.1109/NEMS.2013.6559903
