Title :
Dynamic Micropattern Geometry atop Shape Memory Polymers
Author :
Davis, Kevin A. ; Henderson, J.H.
Author_Institution :
Dept. of Biomed. & Chem. Eng., Syracuse Univ., Syracuse, NY, USA
Abstract :
Substrates micropatterned with cell adhesion proteins have been used to investigate how protein density and geometry affect cell behaviors such as cell migration, growth, and differentiation. Existing technologies are limited in that they typically feature protein micropatterns that are static and unable to change while cells are attached. Here we micropatterned shape memory polymer (SMP) substrates that were capable of transitioning from a stretched state to a contracted state to control the width of patterned lines presented to attached cells. We found that micropattern geometry changed as the SMP substrate transitioned to its unstretched shape. Cells attached to dynamic patterns balled up and contracted their nuclei. The results suggest that micropatterned SMP cell culture substrates can be used to study the temporal aspects of cell mechanobiology.
Keywords :
biomechanics; biomedical materials; cell motility; molecular biophysics; molecular configurations; polymers; proteins; shape memory effects; SMP substrate; SMP substratre; cell adhesion protein; cell behavior; cell differentiation; cell growth; cell mechanobiology; cell migration; contracted state; dynamic micropattern geometry; micropatterned SMP cell culture substrate; micropatterned shape memory polymer substrates; protein density; protein geometry; protein micropattern; shape memory polymers; stretched state; unstretched shape; Adhesives; Geometry; Plastics; Proteins; Shape; Strain; Substrates; micropattern; biomaterial; stem cells; mechanobiology;
Conference_Titel :
Bioengineering Conference (NEBEC), 2013 39th Annual Northeast
Conference_Location :
Syracuse, NY
Print_ISBN :
978-1-4673-4928-4
DOI :
10.1109/NEBEC.2013.160