Quantification of Free Floating and Matrix Bound Growth Factors Secreted by Dynamically-Stimulated Endothelial Cells

Author

Newman, M.R. ; Kazi, N. ; Dumont, C.M. ; Dai, G. ; Thompson, D.M.

Author_Institution

Dept. of Biomed. Eng., Rensselaer Polytech. Inst., Troy, NY, USA

fYear

2013

fDate

5-7 April 2013

Firstpage

88

Lastpage

89

Abstract

Laminar shear stress from fluid flow influences endothelial cell (EC) behavior by increasing the production of specific growth factors (GFs). Moreover, fluid flow affects extracellular matrix (ECM) binding kinetics. This study investigates the effects of fluid flow on ECs for 5 and 12 h. Epithelial GF (EGF) and basic fibroblast GF (bFGF), in both free floating and ECM bound form, were quantified using enzyme-linked immunosorbent assays (ELISAs). Both bound and free floating EGF and bFGF were found to be increased in cultures under flow compared to cultures under static conditions. These results give a comprehensive understanding of the dynamic EC microenvironment and will be used in future studies for manipulation of neural stem cell cultures.

Keywords

biochemistry; biomechanics; cellular biophysics; enzymes; laminar flow; molecular biophysics; ECM bound form; ELISA; basic fibroblast growth factor; dynamically-stimulated endothelial cell; enzyme-linked immunosorbent assays; epithelial growth factor; extracellular matrix binding kinetics; fluid flow effect; free floating EGF; free floating bFGF; laminar shear stress; neural stem cell culture manipulation; static condition; time 12 h; time 5 h; Electronic countermeasures; Fibroblasts; Fluid flow; In vitro; Production; Stem cells; Stress; basic fibroblast growth factor; endothelial cell; epithelial growth factor; extracellular matrix; laminar shear stress;

fLanguage

English

Publisher

ieee

Conference_Titel

Bioengineering Conference (NEBEC), 2013 39th Annual Northeast

Conference_Location

Syracuse, NY

ISSN

2160-7001

Print_ISBN

978-1-4673-4928-4

Type

conf

DOI

10.1109/NEBEC.2013.130

Filename

6574371