Title :
Photodynamic cell membrane perforation for intracellular electrodes
Author :
Shibata, Masatoshi ; Kashiwazaki, Shuhei ; Saito, Takashi K.
Author_Institution :
Takano Co., Ltd., Nagano, Japan
Abstract :
We report a new aspect of rapid (<;10 seconds) light-induced cell membrane perforation for intracellular electrode by Hematoporphyrin (HP), which is an original photosensitizer for photodynamic therapy concept. A microelectrode insertion process to cell could be improved by pinpoint use of the photosensitizing effect to degenerate cell membranes prior to insertion. According to the concept, simple electrode coating with photosensitizer will enable intracellular electrode array for neural engineering.
Keywords :
bioelectric phenomena; biological effects of optical radiation; biomedical electrodes; biomembranes; cellular effects of radiation; microelectrodes; neurophysiology; organic compounds; photochemistry; electrode coating; hematoporphyrin; intracellular electrode array; light-induced cell membrane perforation; microelectrode insertion process; neural engineering; photodynamic cell membrane perforation; photodynamic therapy; photosensitizing effect; Biomembranes; Clamps; Electrodes; Glass; Instruments; Microinjection; Voltage measurement; Animals; Cell Membrane; Electricity; Electrodes; Electrophysiological Phenomena; Hematoporphyrins; Intracellular Space; Microinjections; PC12 Cells; Photochemotherapy; Rats; Signal Processing, Computer-Assisted;
Conference_Titel :
Engineering in Medicine and Biology Society (EMBC), 2013 35th Annual International Conference of the IEEE
Conference_Location :
Osaka
DOI :
10.1109/EMBC.2013.6609623
