Low cost, patterning of human hNT brain cells on parylene-C with UV & IR laser machining

Author

Raos, Brad J. ; Unsworth, Charles P. ; Costa, J.L. ; Rohde, C.A. ; Doyle, C.S. ; Delivopoulos, E. ; Murray, A.F. ; Dickinson, M.E. ; Simpson, M. Cather ; Graham, E.S. ; Bunting, A.S.

Author_Institution

Dept. of Eng. Sci., Univ. of Auckland, Auckland, New Zealand

fYear

2013

fDate

3-7 July 2013

Firstpage

862

Lastpage

865

Abstract

This paper describes the use of 800nm femtosecond infrared (IR) and 248nm nanosecond ultraviolet (UV) laser radiation in performing ablative micromachining of parylene-C on SiO₂ substrates for the patterning of human hNT astrocytes. Results are presented that support the validity of using IR laser ablative micromachining for patterning human hNT astrocytes cells while UV laser radiation produces photo-oxidation of the parylene-C and destroys cell patterning. The findings demonstrate how IR laser ablative micromachining of parylene-C on SiO₂ substrates can offer a low cost, accessible alternative for rapid prototyping, high yield cell patterning.

Keywords

brain; cellular biophysics; high-speed optical techniques; laser applications in medicine; laser beam machining; micromachining; oxidation; IR laser ablative micromachining; IR laser machining; SiO₂ substrates; UV laser machining; femtosecond infrared laser radiation; human hNT astrocytes cell pattering; human hNT brain cell pattering; nanosecond ultraviolet laser radiation; parylene-C; photooxidation; wavelength 248 nm; wavelength 800 nm; Educational institutions; Laser ablation; Laser modes; Strips; Substrates; Astrocytes; Brain; Cell Nucleus; Costs and Cost Analysis; Humans; Infrared Rays; Lasers; Microtechnology; Polymers; Ultraviolet Rays; Xylenes;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology Society (EMBC), 2013 35th Annual International Conference of the IEEE

Conference_Location

Osaka

ISSN

1557-170X

Type

conf

DOI

10.1109/EMBC.2013.6609637

Filename

6609637