Title :
Low cost, patterning of human hNT brain cells on parylene-C with UV & IR laser machining
Author :
Raos, Brad J. ; Unsworth, Charles P. ; Costa, J.L. ; Rohde, C.A. ; Doyle, C.S. ; Delivopoulos, E. ; Murray, A.F. ; Dickinson, M.E. ; Simpson, M. Cather ; Graham, E.S. ; Bunting, A.S.
Author_Institution :
Dept. of Eng. Sci., Univ. of Auckland, Auckland, New Zealand
Abstract :
This paper describes the use of 800nm femtosecond infrared (IR) and 248nm nanosecond ultraviolet (UV) laser radiation in performing ablative micromachining of parylene-C on SiO2 substrates for the patterning of human hNT astrocytes. Results are presented that support the validity of using IR laser ablative micromachining for patterning human hNT astrocytes cells while UV laser radiation produces photo-oxidation of the parylene-C and destroys cell patterning. The findings demonstrate how IR laser ablative micromachining of parylene-C on SiO2 substrates can offer a low cost, accessible alternative for rapid prototyping, high yield cell patterning.
Keywords :
brain; cellular biophysics; high-speed optical techniques; laser applications in medicine; laser beam machining; micromachining; oxidation; IR laser ablative micromachining; IR laser machining; SiO2 substrates; UV laser machining; femtosecond infrared laser radiation; human hNT astrocytes cell pattering; human hNT brain cell pattering; nanosecond ultraviolet laser radiation; parylene-C; photooxidation; wavelength 248 nm; wavelength 800 nm; Educational institutions; Laser ablation; Laser modes; Strips; Substrates; Astrocytes; Brain; Cell Nucleus; Costs and Cost Analysis; Humans; Infrared Rays; Lasers; Microtechnology; Polymers; Ultraviolet Rays; Xylenes;
Conference_Titel :
Engineering in Medicine and Biology Society (EMBC), 2013 35th Annual International Conference of the IEEE
Conference_Location :
Osaka
DOI :
10.1109/EMBC.2013.6609637
