Title :
Effects of low amplitude pulsed radiofrequency stimulation with different waveform in rats for neuropathic pain
Author :
Lin, W.T. ; Chang, C. Hwa ; Cheng, C.Y. ; Chen, Meng Chang ; Wen, Y.R. ; Lin, C.T. ; Lin, C.W.
Author_Institution :
Grad. Inst. of Biomed. Electron. & Bioinf., Nat. Taiwan Univ., Taipei, Taiwan
Abstract :
Pulsed-radiofrequency (PRF) electrical stimulation has been widely used for chronic pain treatment. It has been demonstrated with advantages of low temperature over traditional continuous radiofrequency (CRF) lesions with higher amplitude and mono polar electrode to treat pain in clinics (frequency 500 KHz, Pulse duration 20 msec, Amplitude 45 V, Treatment 2 min). We compare the effects of different pulse waveforms and PRF parameters (Pulse duration 25 ms, Treatment duration 5 min, low amplitude of 2.5/1.25 V) with a miniature bi-polar electrode on Dorsal root ganglion (DRG). The pain relief effect due to PRF is evaluated by using Von Frey method for the pain threshold index based on behavior response to mechanical stimulus of various strengths. Experimental results of Von Frey Score show that the sinusoidal group has higher responses than the square wave one. Both fast and secondary expressed proteins of c-fos and pp38 are measured from spinal cord tissue sectioning slides to characterize the pain associated inflammatory responses and their responses due to PRF stimulation.
Keywords :
bioelectric phenomena; biological tissues; biomechanics; biomedical electrodes; mechanical strength; medical disorders; molecular biophysics; neurophysiology; patient treatment; proteins; Dorsal root ganglion; PRF parameters; PRF stimulation; Von Frey Score; Von Frey method; c-fos; chronic pain treatment; fast expressed proteins; frequency 500 kHz; low amplitude pulsed radiofrequency electrical stimulation effects; mechanical stimulus; miniature bipolar electrode; monopolar electrode; neuropathic pain; pain associated inflammatory responses; pain relief effect; pain threshold index; pulse waveform effects; secondary expressed proteins; sinusoidal group; spinal cord tissue sectioning slides; square wave; time 20 min; time 5 min; traditional continuous radiofrequency lesions; voltage 45 V; Electrodes; Neuropathic pain; Proteins; Radio frequency; Rats; Surgery;
Conference_Titel :
Engineering in Medicine and Biology Society (EMBC), 2013 35th Annual International Conference of the IEEE
Conference_Location :
Osaka
DOI :
10.1109/EMBC.2013.6610319
