Title :
High throughput cell sorting device using dielectrophoresis and fluid-induced shear force
Author :
Takahashi, Y. ; Takeuchi, Shoji ; Miyata, Sumiko
Author_Institution :
Dept. of Mech. Eng., Keio Univ., Yokohama, Japan
Abstract :
Cell sorting is important technology in many areas such as medical diagnosis in hospitals and cell engineering. Dielectrophoresis (DEP) is one of the promising approaches for cell separation because it does not require any fluorescent dye or antibody. In our previous study, we developed DEP cells sorting device, however the amount of throughput was not sufficient. In this study, we developed high throughput cell sorting device using dielectrophoretic and fluid-induced shear force. As the pilot study, mixed solution containing bovine chondrocytes and polystyrene beads were precisely separated using our novel DEP cell sorting device.
Keywords :
bioMEMS; cellular biophysics; dyes; electrophoresis; microfluidics; tissue engineering; DEP cells; bovine chondrocytes; cell separation; cell sorting device; dielectrophoresis; fluid-induced shear force; mixed solution; polystyrene beads; Dielectrophoresis; Electric fields; Electrodes; Force; Sorting; Suspensions; Throughput;
Conference_Titel :
Engineering in Medicine and Biology Society (EMBC), 2013 35th Annual International Conference of the IEEE
Conference_Location :
Osaka
DOI :
10.1109/EMBC.2013.6610538