A bioinformatics pipeline for sequence to structure: A case study with a Cml patient undergoing treatment with imatinib

Chronic Myeloid Leukaemia (CML) is a resultant of the 9:22 translocation event leading to the constitutive kinase activity of BCR-ABL. Imatinib is the drug used as the first line therapy in CML. We report a longitudinal case study for a CML patient under treatment with imatinib. The bone marrow aspirate of this CML patient was used for exome sequencing. Single nucleotide variants (SNVs) unique to the exome sequencing sample datasets were analysed with an emphasis on kinases. These mutations were mapped to the structure to further understand the significance of the SNVs in the context of its stability and kinase-drug interaction. Here we present a data filtering pipeline with examples for sequence to structure approach. This strategy can be used to filter kinases from next generation sequencing data relevant to cancers.