Can Terahertz time-domain spectroscopy detect an extended hydration layer around peptides?

Author

Ding, Tianyu ; Middelberg, A.P.J. ; Bye, J.M. ; Falconer, R.J.

Author_Institution

CVRI, Nat. Univ. of Singapore, Singapore, Singapore

fYear

2013

fDate

1-6 Sept. 2013

Firstpage

1

Lastpage

2

Abstract

A peptide was added to a KF solution and the absorbance measured by Terahertz time-domain spectroscopy (THz-TDS). The absorbance dropped then plateaued at ~25 mM. This is consistent with a hydration layer around 11-17 Å thick with lower absorbance than bulk water. Terahertz spectroscopy using synchrotron light performed on a solution containing the protein bovine serum albumin (BSA) confirmed the rise in absorbance observed using p-germanium spectroscopy and the plateauing observed by THz-TDS in aqueous peptide samples. Using the inflection in the decline of the absorbance, the thickness of the hydration layer around BSA can be estimated at 15A thick.

Keywords

biochemistry; molecular biophysics; proteins; solvation; synchrotrons; terahertz wave spectra; time-domain analysis; BSA; KF solution; THz-TDS; aqueous peptide samples; hydration layer; p-germanium spectroscopy; protein bovine serum albumin; synchrotron light; terahertz time-domain spectroscopy;

fLanguage

English

Publisher

ieee

Conference_Titel

Infrared, Millimeter, and Terahertz Waves (IRMMW-THz), 2013 38th International Conference on

Conference_Location

Mainz

Type

conf

DOI

10.1109/IRMMW-THz.2013.6665563

Filename

6665563