Title :
Probing label free antibody interactions with HA protein using terahertz pulsed spectroscopy
Author :
Yiwen Sun ; Jian Zuo ; Zhenwei Zhang ; Cunlin Zhang ; Zexuan Zhu
Author_Institution :
Dept. of Biomed. Eng., Shenzhen Univ., Shenzhen, China
Abstract :
In this paper we use terahertz spectroscopy to study the concentration dependence non-linear absorption behaviors of the influenza virus hemagglutinin (HA) protein and effects of HA protein interacting with the neutralization antibody in the solutions. The thickness of the hydration shell around the HA protein was determined based on two and three components model. The absorption coefficient was measured over the frequency range 0.1-1.5THz. The terahertz properties of HA was strongly affected by the presence of a specific antibody. Therefore terahertz spectroscopy may be useful for label-free influenza virus detection in the future.
Keywords :
absorption coefficients; biochemistry; biomolecular effects of radiation; microorganisms; proteins; solvation; spectrochemical analysis; terahertz wave spectra; HA protein; absorption coefficient; concentration dependence nonlinear absorption behaviors; frequency 0.1 THz to 1.5 THz; hydration shell thickness; influenza virus hemagglutinin protein; label free antibody interactions; label-free influenza virus detection; neutralization antibody; specific antibody; terahertz pulsed spectroscopy; Absorption; Biomedical imaging; Educational institutions; Influenza; Protein engineering; Proteins; Spectroscopy;
Conference_Titel :
Infrared, Millimeter, and Terahertz Waves (IRMMW-THz), 2013 38th International Conference on
Conference_Location :
Mainz
DOI :
10.1109/IRMMW-THz.2013.6665566
