Title :
Raman and Surface-Enhanced Raman Scattering (SERS) Studies of the Thrombin-Binding Aptamer
Author :
Tsai-Chin Wu ; Vasudev, M. ; Dutta, Maitreyee ; Stroscio, Michael
Author_Institution :
Bioeng. Dept., Univ. of Illinois, Chicago, IL, USA
Abstract :
Surface-enhanced Raman scattering is used to study the Raman spectra and peak shifts the thrombin-binding aptamer (TBA) on substrates having two different geometries; one with a single stranded sequence and one with double stranded sequence. The Raman signals of the deoxyribonucleic acids on both substrates are enhanced and specific peaks of bases are identified. These results are highly reproducible and have promising applications in low cost nucleic acid detection.
Keywords :
DNA; biochemistry; biological techniques; enzymes; molecular biophysics; molecular configurations; surface enhanced Raman scattering; Raman signals; Raman spectra; deoxyribonucleic acids; double stranded sequence; low cost nucleic acid detection; molecular geometry; peak shifts; single stranded sequence; substrates; surface-enhanced Raman scattering study; thrombin-binding aptamer; Aptamer; DNA; nanobiotechnology; nanoparticle; surface-enhanced Raman scattering; Aptamers, Nucleotide; DNA; Gold; Metal Nanoparticles; Potassium; Spectrum Analysis, Raman;
Journal_Title :
NanoBioscience, IEEE Transactions on
DOI :
10.1109/TNB.2013.2242484
