Title :
Microfluidic biochip for phytoplankton cell counting
Author :
Matiolli Machado, Leandro ; Mattiello, Izadora ; Mendes Lopes, Rubens ; Carreno, Marcelo Nelson Paez
Author_Institution :
Lab. de Microeletronica, USP, São Paulo, Brazil
Abstract :
We present a prototype of a PDMS-based microfluidic device for automatic counting of phytoplankton cells in culture solution. The device functioning is based on monitoring changes in the intensity of a cross-channel fiber optical signal during downstream cell transit. The microfluidc chip is fabricated by PDMS soft lithography technique, and the light source and the detection unit are commercial LED and photodiodes integrated to the microfluidic device. The results demonstrate the reliability of the automatic counting experiments as compared with a manual microscopic counting method.
Keywords :
bioMEMS; biological techniques; botany; cellular biophysics; lab-on-a-chip; light emitting diodes; light sources; microfabrication; microfluidics; optical fibres; photodiodes; soft lithography; PDMS soft lithography technique; PDMS-based microfluidic device; automatic counting; commercial LED; cross-channel fiber optical signal intensity; culture solution; detection unit; device functioning; downstream cell transit; light source; manual microscopic counting method; microfluidic biochip; photodiodes; phytoplankton cell counting; Microfluidics; automatic phytoplankton counting; biochips;
Conference_Titel :
Microelectronics Technology and Devices (SBMicro), 2013 Symposium on
Conference_Location :
Curitiba
Print_ISBN :
978-1-4799-0516-4
DOI :
10.1109/SBMicro.2013.6676146
