Electroporation system generating wide range square-wave pulses for biological applications

Author

Stankevic, Voitech ; Novickij, Vitalij ; Balevicius, Saulius ; Zurauskiene, Nerija ; Baskys, Algirdas ; Dervinis, A. ; Bleizgys, V.

Author_Institution

Center for Phys. Sci. & Technol., Vilnius Gediminas Tech. Univ., Vilnius, Lithuania

fYear

2013

fDate

Oct. 31 2013-Nov. 2 2013

Firstpage

33

Lastpage

36

Abstract

The design of a wide range square-wave pulse electroporation system is presented. The system is able to generate pulses having voltage amplitudes up to 4000 V and currents up to 100 A. The quantity of accumulated energy is optimized by the use of the variable capacitor bank. The proposed device has an integrated graphic LCD and measurement modules for current pulse visualization, and does not require an external oscilloscope. The whole system was tested with suspension of human lymphoma cells of Jurkat line in RPMI medium under a variety of electroporation conditions.

Keywords

biological techniques; capacitors; cellular biophysics; liquid crystal displays; Jurkat line; RPMI medium; accumulated energy; biological applications; capacitor bank; current pulse visualization; electroporation conditions; human lymphoma cells; integrated graphic LCD; voltage amplitudes; wide range square-wave pulse electroporation system; Biology; Capacitors; Energy storage; Resistance; Suspensions; Transistors; Voltage measurement;

fLanguage

English

Publisher

ieee

Conference_Titel

Biomedical Circuits and Systems Conference (BioCAS), 2013 IEEE

Conference_Location

Rotterdam

Type

conf

DOI

10.1109/BioCAS.2013.6679633

Filename

6679633