Raman mapping studies of human ectocervical tissue

Author

Daniel, Andy ; Arunal, P. ; Ganesan, S. ; Koteeswaran, D. ; Murali Krishna, C. ; David, Barak

Author_Institution

Dept. of Med. Phys., Anna Univ., Chennai, India

fYear

2013

fDate

28-30 Oct. 2013

Firstpage

105

Lastpage

107

Abstract

In this pilot study, the micro Raman mapping of normal cervical tissue was carried out to understand the biomolecular alteration at different regions of the cervical tissue. The constructed Raman mapping was compared with respect to different layers of cervical tissue viz., Superficial layer, Intermediate layer, Parabasal layer, Basal layer and underlying Connective tissue through the averaged Raman spectrum at the respective sites. Raman spectra collected from the basal side of epithelium consistently showed higher DNA contributions and lower lipid contributions when compared with superficial epithelium spectra. Spectra of the underlying connective tissue exhibited a strong contribution of collagen and elastin fibrils.

Keywords

DNA; Raman spectra; Raman spectroscopy; biological tissues; molecular biophysics; patient diagnosis; proteins; Basal layer; DNA contribution; Intermediate layer; Parabasal layer; Raman mapping studies; Superficial layer; averaged Raman spectrum; biomolecular alteration; collagen; connective tissue; elastin fibrils; epithelium basal side; human ectocervical tissue; lipid contribution; microRaman mapping; normal cervical tissue; superficial epithelium spectra; Pathology; Raman spectroscopy; Uterine cervix; microspectrocopic mapping;

fLanguage

English

Publisher

ieee

Conference_Titel

Photonics (ICP), 2013 IEEE 4th International Conference on

Conference_Location

Melaka

Print_ISBN

978-1-4673-6073-9

Type

conf

DOI

10.1109/ICP.2013.6687082

Filename

6687082