Title :
Broadband microchamber for electrical detection of live and dead biological cells
Author :
Palego, Cristiano ; Merla, Caterina ; Ning, Yaqing ; Multari, Caroline R. ; Cheng, Xiaoyin ; Molinero, D.G. ; Ding, Guoru ; Luo, Xiaohua ; Hwang, James C. M.
Author_Institution :
Lehigh Univ., Bethlehem, PA, USA
Abstract :
A novel broadband microchamber for electrical detection of live and dead biological cells was designed, fabricated and tested. The microchamber was formed between a gold coplanar waveguide fabricated on a quartz slide and the microfluidic channels fabricated in a polydimethylsiloxane cover. The coplanar waveguide allowed broadband impedance matching and efficient cell trapping. The microfluidic channels delivered single cells precisely. Tests on Jurkat cells in both time and frequency domains showed that live cells had lower resistance but higher capacitance than that of dead cells.
Keywords :
bioMEMS; bioelectric phenomena; biological techniques; capacitance; cellular biophysics; coplanar waveguides; electrical resistivity; gold; impedance matching; microfabrication; microfluidics; Au; Jurkat cells; broadband impedance matching; broadband microchamber; capacitance; cell trapping; dead biological cells; electrical detection; frequency domains; gold coplanar waveguide; live biological cells; microfluidic channels; polydimethylsiloxane cover; quartz slide; resistance; time domains; Broadband communication; Capacitance; Charge carrier processes; Electrical resistance measurement; Microchannel; Microfluidics; Microwave measurement; Biological cells; bioimpedance; biosensors; microfluidics; microsensor; reflectometry;
Conference_Titel :
Microwave Symposium Digest (IMS), 2013 IEEE MTT-S International
Conference_Location :
Seattle, WA
Print_ISBN :
978-1-4673-6177-4
DOI :
10.1109/MWSYM.2013.6697355
