Broadband microchamber for electrical detection of live and dead biological cells

Author

Palego, Cristiano ; Merla, Caterina ; Ning, Yaqing ; Multari, Caroline R. ; Cheng, Xiaoyin ; Molinero, D.G. ; Ding, Guoru ; Luo, Xiaohua ; Hwang, James C. M.

Author_Institution

Lehigh Univ., Bethlehem, PA, USA

fYear

2013

fDate

2-7 June 2013

Firstpage

1

Lastpage

3

Abstract

A novel broadband microchamber for electrical detection of live and dead biological cells was designed, fabricated and tested. The microchamber was formed between a gold coplanar waveguide fabricated on a quartz slide and the microfluidic channels fabricated in a polydimethylsiloxane cover. The coplanar waveguide allowed broadband impedance matching and efficient cell trapping. The microfluidic channels delivered single cells precisely. Tests on Jurkat cells in both time and frequency domains showed that live cells had lower resistance but higher capacitance than that of dead cells.

Keywords

bioMEMS; bioelectric phenomena; biological techniques; capacitance; cellular biophysics; coplanar waveguides; electrical resistivity; gold; impedance matching; microfabrication; microfluidics; Au; Jurkat cells; broadband impedance matching; broadband microchamber; capacitance; cell trapping; dead biological cells; electrical detection; frequency domains; gold coplanar waveguide; live biological cells; microfluidic channels; polydimethylsiloxane cover; quartz slide; resistance; time domains; Broadband communication; Capacitance; Charge carrier processes; Electrical resistance measurement; Microchannel; Microfluidics; Microwave measurement; Biological cells; bioimpedance; biosensors; microfluidics; microsensor; reflectometry;

fLanguage

English

Publisher

ieee

Conference_Titel

Microwave Symposium Digest (IMS), 2013 IEEE MTT-S International

Conference_Location

Seattle, WA

ISSN

0149-645X

Print_ISBN

978-1-4673-6177-4

Type

conf

DOI

10.1109/MWSYM.2013.6697355

Filename

6697355