Single Cell Microdosimetric Studies Comparing Ideal and Measured Nanosecond Pulsed Electric Fields

Recently, the promising effects induced by pulsed electric fields with high intensity and short duration have been highlighted. At the nanosecond time scale, electric pulse targets become both the plasmatic membrane and the sub-cellular structures (possibility of intracellular manipulation). In this paper, a circuit cell model with nucleus is presented, validated and used in order to assess the different cellular and sub-cellular (i.e. nucleus) effects, comparing ideal nanosecond pulsed electric fields (nsPEFs) waveforms with the ones measured from a planar, broadband matched microchamber.